Pengaruh Campuran Ekstrak Etanol Buah Pare dan Biji Pinang Terhadap Kadar Testosteron Tikus dengan Menggunakan Metoda ELISA
-
Vivi Sofia
*
Universitas Tjut Nyak Dhien, Medan,
Indonesia
- Salsabila Rahmawanti Universitas Ahmad Dahlan, Yogyakarta, Indonesia (*) Corresponding Author
Abstract
Testosterone is a hormone that plays an important role in sexual development and reproductive function. Infertility problems, decreased libido and erectile dysfunction are closely related to decreased testosterone levels in the blood. This study aims to see the effect of a mixture of ethanol extracts of bitter melon (Momordica charantia L.) and areca nut (Areca catechu L) on testosterone levels in male white rats. This study used the Sexual Behavior method. 30 Wistar rats were divided into 5 groups consisting of distilled water control, positive control (X-Gra®) 45 mg/kg BW, ethanol extract of bitter melon (EEBP) dose of 100 mg/kg BW, ethanol extract of areca nut (EEP) dose of 50 mg/kg BW and mixed group of EEBP:EEP (1:1), after 15 days, blood samples were taken and testosterone levels were analyzed. The data obtained were statistically analyzed with one-way ANOVA test and continued with Tukey's HSD test with 95% confidence level (p<0.05) using SPSS ver.20 software. The results showed that testosterone levels in the EEBP group at a dose of 100 mg / kg BW and a mixture of EEBP: EEP (1: 1) of 2.31 ± 0.84 ng/ml and 1.65 ± 0.54 ng/ml were not significantly different when compared to the distilled water control group (p < 0.05). Testosterone levels in the EEP group at a dose of 50 mg/kg BW of 3.43 ± 0.98 ng/ml showed a significant difference with the aquades control group (p<0.05). The mixture of bitter melon ethanol extract at a dose of 100 mg/kg BW and areca nut seeds at a dose of 50 mg/kg BW was not able to increase testosterone levels, while the ethanol extract of areca nut seeds given singly at a dose of 50 mg/kg BW could increase testosterone hormone levels in male white rats. Based on the results obtained in this study, it can be concluded that a mixture of bitter melon ethanol extract at a dose of 100 mg / kg BW and areca nut seeds at a dose of 50 mg / kg BW is not able to increase testosterone levels, while areca nut ethanol extract given singly at a dose of 50 mg / kg BW can increase testosterone levels in male white rats.
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References
Adedokun, O., Gbolade, A., & Ayinde, B. (2022). 13, 14-Epoxyoleanan-3-ol-acetate: A Male Fertility-Enhancing Constituent from Hexane Fraction of Momordica charantia L. (Cucurbitaceae). Turkish Journal of Pharmaceutical Sciences, 19(2), 180–186. https://doi.org/10.4274/tjps.galenos.2021.48264
Akmal, M., Adam, M., Toras, M., & Triva Murtina Lubis (2015). The Effect of Pegagan Leaf Exstract (Centella asiatica (L.) Urban) Administration on Testosterone Concentration of Male White Rats (Rattus norvegicus). Jurnal Medika Veterinaria, 9(1); 40-43.
Ansari, A., Mahmood, T., Bagga, P., Ahsan, F., Shamim, A., Ahmad, S., Shariq, M., & Parveen, S. (2021). Areca catechu: A phytopharmacological legwork. In Food Frontiers (Vol. 2, Issue 2, pp. 163–183). John Wiley and Sons Inc. https://doi.org/10.1002/fft2.70
Barone, B., Napolitano, L., Abate, M., Cirillo, L., Reccia, P., Passaro, F., Turco, C., Morra, S., Mastrangelo, F., Scarpato, A., Amicuzi, U., Morgera, V., Romano, L., Calace, F. P., Pandolfo, S. D., De Luca, L., Aveta, A., Sicignano, E., Trivellato, M., Crocetto, F. (2022). The Role of Testosterone in the Elderly: What Do We Know? In International Journal of Molecular Sciences (Vol. 23, Issue 7). MDPI. https://doi.org/10.3390/ijms23073535
Birowo, P., Deswanto, I. A., & Rasyid, N. (2019). Epidemiology of erectile dysfunction: A cross-sectional web-based survey conducted in an Indonesian national referral hospital. F1000Research, 8, 817. https://doi.org/10.12688/f1000research.18930.1
Brunetti, P., Lo Faro, A. F., Tini, A., Busardò, F. P., & Carlier, J. (2020). Pharmacology of herbal sexual enhancers: A review of psychiatric and neurological adverse effects. In Pharmaceuticals (Vol. 13, Issue 10, pp. 1–52). MDPI AG. https://doi.org/10.3390/ph13100309
Djohari, M., Fernando, A., SAFITRI Sekolah Tinggi Ilmu Farmasi Riau, A., Kamboja, J., & Baru Panam, S. (2020). Aktivitas Daya Hambat Ekstrak Etanol Biji Pinang (Areca catechu L.) Terhadap Isolat Bakteri Gigi (Inhibition Activity Of Ethanol Extract Of Areca catechu L. Against Isolation Of Tooth’s Bacteria). JURNAL ILMU KEFARMASIAN INDONESIA, 18(1), 81–87.
Hendry, Z., & Fahmi, R. (2023). Effect of giving Extract Ethanol from Bitter Gourd Fruit (Momordica Charantia L) on the Quality and Quantity of Mouse Spermatozoa (Mus Musculus). International Journal of Medicine and Health (IJMH), 2(3). https://doi.org/10.55606/ijmh.v2i2.2195
Ide, H. (2023). State-of-the-Art Review in Endocrinology (Vol. 2023, Issue 7).
Lee, K. S., Kim, H. P., Park, H. J., Young & Yoon, G. (2021). Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats. https://doi.org/10.1007/s10068
Maidy Belanita, P. (2023). PEMBERIAN EKSTRAK ETANOL BUAH PARE (Momordica charantia L.) MENURUNKAN KUALITAS SPERMATOZOA PADA TIKUS WISTAR (Rattus norvegicus). Jurnal Keperawatan Suaka Insan (JKSI), 8(2).
Medicina, B., Totong Kamaluddin, M., & Parisa, N. (2019). Aphrodisiac Effects of Areca Fruit in Erectile Dysfunction Rat Model. In Bioscientia Medicina (Vol. 3, Issue 3). www.bioscmed.com
Michaelle, C., Cheikna, Z., Durand, D.-N., Mihaela, C., Pacôme, N., Ioana, O. G., Bianca, F., Aly, S., Rodica, M. D., Lamine, B.-M., & Farid, B.-M. (2020). Phytochemical screening and antimicrobial activity of Momordica charantia L. and Morinda lucida Benth extracts from Benin. African Journal of Microbiology Research, 14(8), 426–435. https://doi.org/10.5897/ajmr2020.9347
Santoso, H., & Zen, S. (2023). PROSIDING SNPPM-5 (Seminar Nasional Penelitian dan Pengabdian kepada Masyarakat) Tahun 2023 Universitas Muhammadiyah Metro Halaman | 64 BIOPROSPEKSI TANAMAN APHRODISIAK INDONESIA. https://prosiding.ummetro.ac.id/index.php/snppm
Sarapi, V. A., Bodhi, W., & Citraningtyas, G. (2015). UJI EFEK AFRODISIAK EKSTRAK ETANOL BUAH PARE (Momordica charantia L.) TERHADAP LIBIDO TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus). In Jurnal Ilmiah Farmasi, 4(3).
Setiawan, H., & Subagja, R. A. (2023). Pengaruh Pemberian Jus Pare (Momordica charantia L.) Terhadap Kualitas Sperma dan Histologi Testis Tikus Wistar. Al-Kauniyah: Jurnal Biologi, 17(1), 10–20. https://doi.org/10.15408/kauniyah.v16i2.1.23216
Sofia, V., & Saiful Bachri, M. (2023). Pengaruh Campuran Ekstrak Etanol Pare (Momordica charantia L.) dan Pinang (Areca catechu L.) Terhadap Kadar e-NOS Tikus Putih. Jurnal Farmasi Higea, 15(1). www.jurnalfarmasihigea.org
Soliman, G. A., Abdel-Rahman, R. F., Ogaly, H. A., Althurwi, H. N., Abd-Elsalam, R. M., Albaqami, F. F., & Abdel-Kader, M. S. (2020). Momordica charantia Extract Protects against Diabetes-Related Spermatogenic Dysfunction in Male Rats: Molecular and Biochemical Study. Molecules, 25(12). https://doi.org/10.3390/MOLECULES25225255
Svobodova, B., Barros, L., Calhelha, R. C., Heleno, S., Alves, M. J., Walcott, S., Bittova, M., Kuban, V., & Ferreira, I. C. F. R. (2017). Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago. Industrial Crops and Products, 95, 365–373. https://doi.org/10.1016/j.indcrop.2016.10.046
Tumkiratiwong, P., Ploypattarapinyo, R., Pongchairerk, U., & Thong-asa, W. (2014). Reproductive toxicity of Momordica charantia ethanol seed extracts in male rats. In Iran J Reprod Med, 12(10)
Verma, D., Shrivastav, T. G., & Thakur, S. C. (2022). Homologous ELISA for detection of 17α-methyltestosterone in serum. International Journal of Health Sciences, 6(6), 6457–6474. https://doi.org/10.53730/ijhs.v6ns6.11036
Wang, X., Huang, L., Zhao, D., Qiu, J., & Ma, Y. (2024). Research on the Changes in Catecholamine and Other Indicators during Different Training Phases in Female Volleyball Players. ORIGINAL RESEARCH, 30(7)
Zhao, G., Zhang, S., Zou, Y., Jia, N., Zheng, L., & Dong, J. (2025). Detection of testosterone based on the interaction between variable regions of antibody heavy chain and light chain. Frontiers in Bioengineering and Biotechnology, 13. https://doi.org/10.3389/fbioe.2025.1499164
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