Analisa Fitokimia dan Aktivitas Antioksidan Sirup Herbal Ekstrak Buah Labu (Curcubita Moschata) dan Rimpang Kunyit (Curcuma Domestica) dengan Metode DPPH
- Khamdiyah Indah Kurniasih Universitas Harapan Bangsa, Purwokerto, Indonesia
-
Fauziah Fauziah
*
Universitas Harapan Bangsa, Purwokerto,
Indonesia
- Misworo Misworo Universitas Harapan Bangsa, Purwokerto, Indonesia
- Hansen Prismadito Universitas Harapan Bangsa, Purwokerto, Indonesia (*) Corresponding Author
Abstract
Pumpkin is one of the natural ingredients with antioxidant properties, and it helps reduce symptoms of diabetes, hypertension, tumors, cholesterol, antibacterial, anthelmintic, and anti-inflammatory effects. The polyphenol and flavonoid contents in pumpkin are 97.4 mgGAE/gram and 28.6 mgQE/gram, respectively, with high antioxidant activity against DPPH radicals at 0.065 ± 0.010 mol TE/gram. Turmeric rhizome is also a plant with high antioxidant activity. Turmeric rhizome contains curcumin as the main active compound, with a content of 7.798%. The ethanol extract of turmeric rhizome exhibits antioxidant activity with an IC50 value of 41.95 µg/mL (DPPH). Studies on the composition and benefits of pumpkin and turmeric rhizome have been conducted. However, so far, there has been no standardization of the simplicia and extracts of these two plants from Central Java. The herbal syrup combining pumpkin and turmeric extracts is an herbal product that has not been scientifically proven regarding its metabolite compounds and antioxidant activity, which play a role in addressing various degenerative diseases. Therefore, standardization of the simplicia and verification of the phytochemical metabolites and antioxidant content are necessary. This study was conducted by comparing the IC50 values of three syrup formulas: turmeric rhizome syrup (F1), pumpkin syrup (F2), and a mixture of pumpkin and turmeric rhizome syrup (F3). The results showed that Formula 1 (F1) had the lowest IC50 value of 17.366 µg/mL, indicating the highest antioxidant potential, followed by F3 with an IC50 of 47.862 µg/mL, and F2 with the lowest antioxidant activity, with an IC50 value of 288.545 µg/mL.
Downloads
References
Amin, S., Cahya, N. A., Rachmat, S. W., Adri, A., & Amelya, I. N. (2024). Tanaman Lingkungan Sebagai Penyembuhan Penyakit. PT. Sonpedia Publishing Indonesia.
Astutik, L., & Yanti, E. F. (2023). The Effect of Pumpkin Fruit Ripeness (Cucurbita moschata. D) on Total Flavonoid Levels and Antioxidant Activity. Biology, Medicine, & Natural Product Chemistry, 12(1), 17–23.
Bachtiar, A. R. (2023). Penetapan Kadar Flavonoid Total Buah Dengen (Dillenia serrata) Menggunakan Metode Spektrofotometri UV-Vis. Makassar Natural Product Journal (MNPJ), 86–101.
Budiman, H., Supriningrum, R., & Sundu, R. (2024). KARAKTERISASI DAN SKRINING FITOKIMIA BUAH LABU KUNING (Cucurbita moschata Duch.). Jurnal Riset Kefarmasian Indonesia, 6(1), 16–36.
Fatmawati, A., & NP, A. (2019). Penetapan kadar flavonoid total ekstrak etanol daun kelor (moringa oleifera lam) dengan metode kromatografi lapis tipis densitometri. Proc Conf Published Online, 1–7.
Indiarto, R., & Rezaharsamto, B. (2020). The physical, chemical, and microbiological properties of peanuts during storage: A review. Int. J. Sci. Technol. Res, 9(3), 1909–1913.
Jadhao, A. G., Sanap, M. J., Patil, P. A., & others. (2021). Formulation and evaluation of herbal syrup. Asian Journal of Pharmaceutical Research and Development, 9(3), 16–22.
Jambak, K., Nainggolan, M., & Dalimunthe, A. (2019). Antioxidant activity of ethanolic extract and n-hexane fraction from sikkam (Bischofia javanica Blume) stem bark. Asian Journal of Pharmaceutical Research and Development, 7(2), 1–5.
Marbun, N., Sitorus, P., & Sinaga, S. M. (2018). Antidiabetic effects of pumpkin (Cucurbita moschata durch) flesh and seeds extracts in streptozotocin induced mice. Asian J Pharm Clin Res, 11(2), 2018.
Men, X., Choi, S.-I., Han, X., Kwon, H.-Y., Jang, G.-W., Choi, Y.-E., Park, S.-M., & Lee, O.-H. (2021). Physicochemical, nutritional and functional properties of Cucurbita moschata. Food Science and Biotechnology, 30, 171–183.
Mokhtar, K. S., Rahman, N. A., Haron, M. S., & Abd Rahman, N. H. (2020). An exploration of disease awareness among tuberculosis patients: The empirical link between attitude and self-preventive care. The Journal of Infection in Developing Countries, 14(04), 349–359.
Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, e47.
Parham, S., Kharazi, A. Z., Bakhsheshi-Rad, H. R., Nur, H., Ismail, A. F., Sharif, S., RamaKrishna, S., & Berto, F. (2020). Antioxidant, antimicrobial and antiviral properties of herbal materials. Antioxidants, 9(12), 1309.
Priori, D., Valduga, E., Villela, J. C. B., Mistura, C. C., Vizzotto, M., Valgas, R. A., & Barbieri, R. L. (2016). Characterization of bioactive compounds, antioxidant activity and minerals in landraces of pumpkin (Cucurbita moschata) cultivated in Southern Brazil. Food Science and Technology (Campinas), 37(1), 33–40.
Purba, A. (2019). Identifikasi Kadar Fenol dan Flavonoid Ekstrak Etanol Rimpang Kunyit (Curcuma longa L.). Herbal Medicine Journal, 2(1), 18–24.
Purwanto, D., Bahri, S., & Ridhay, A. (2017). Uji aktivitas antioksidan ekstrak buah purnajiwa (Kopsia arborea Blume.) dengan berbagai pelarut. KOVALEN: Jurnal Riset Kimia, 3(1), 24–32.
Ramadhan, M. F., Supriani, S., Supriani, W. Y. S., Khotimah, K., & Setyaningsih, M. (2024). Uji Fitokimia Ekstrak Etanol 96% dan Fraksi Air, Fraksi Kloroform serta Fraksi N-Hexana Rimpang Kunyit (Curcuma Longa L). Jurnal Farmasetis, 13(2), 71–78.
Ramonah, D. (2023). Edukasi dan Pemanfaatan Labu Kuning sebagai Bahan Pangan Sehat di Desa Wisata Jamalsari. REPOSITORY STIFAR.
Sadler, M. J. (2014). Foods, Nutrients and Food Ingredients with Authorised EU Health Claims: Volume 1. Elsevier.
Septiana, A. T., Samsi, M., & Mustaufik, M. (2017). Pengaruh penambahan rempah dan bentuk minuman terhadap aktivitas antioksidan berbagai minuman tradisional Indonesia. Agritech, 37(1), 7–15.
Sies, H. (1997). Oxidative stress: Oxidants and antioxidants. Experimental Physiology: Translation and Integration, 82(2), 291–295.
Skoog, D. A., Holler, F. J., & Crouch, S. R. (2019). Textbook" principles of instrumental analysis.". Cengage Learning. Core. Ac. Uk. Https://Core. Ac. Uk/Download/Pdf/232277508. Pdf.
Sridhar, K., & Charles, A. L. (2019). In vitro antioxidant activity of Kyoho grape extracts in DPPH and ABTS assays: Estimation methods for EC50 using advanced statistical programs. Food Chemistry, 275, 41–49.
Suprihatin, T., Rahayu, S., Rifa’i, M., & Widyarti, S. (2020). Senyawa pada serbuk rimpang kunyit (Curcuma longa L.) yang berpotensi sebagai antioksidan. Buletin Anatomi Dan Fisiologi, 5(1), 35–42.
Triyono, T., Chaerunisaa, A. Y., & Subarnas, A. (2018). Antioxidant activity of combination ethanol extract of turmeric rhizome (Curcuma domestica Val) and ethanol extract of trengguli bark (Cassia Fistula L) with DPPH Method. Indonesian Journal of Pharmaceutical Science and Technology, 5(2), 43–48.
Bila bermanfaat silahkan share artikel ini
Berikan Komentar Anda terhadap artikel Analisa Fitokimia dan Aktivitas Antioksidan Sirup Herbal Ekstrak Buah Labu (Curcubita Moschata) dan Rimpang Kunyit (Curcuma Domestica) dengan Metode DPPH
Pages: 1-7
Copyright (c) 2025 Khamdiyah Indah Kurniasih, Fauziah Fauziah, Misworo Misworo, Hansen Prismadito
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (Refer to The Effect of Open Access).
