Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi
-
Nasri Nasri
*
Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior, Medan,
Indonesia
http://orcid.org/0000-0002-5441-6920
- Vera Estefania Kaban Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior, Medan, Indonesia
- Denny Satria Universitas Sumatera Utara, Medan, Indonesia
- Hariyadi Dharmawan Syahputra Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior, Medan, Indonesia
- Zulmai Rani Universitas Muslim Nusantara AL-Washliyah, Medan, Indonesia (*) Corresponding Author
Abstract
Infectious diseases are conditions that can be brought on by a variety of different organisms, including bacteria, viruses, fungi, and parasites. Typhoid fever is an example of an infectious disease that frequently causes the body's defenses to come under attack. The fever associated with typhoid is a leading cause of death around the world. Infection with the bacterium Salmonella typhi is considered to be one of the potential causes of typhoid fever. When antibiotics are used to treat bacterial infections, there is a risk that the bacteria will become resistant to the medication. In day-to-day life, basil leaves are utilized both as a cooked vegetable and as a fresh vegetable. The antibacterial properties of kemangi leaves have yet to be fully uncovered. The purpose of this study was to investigate the various mechanisms that are involved in antibacterial activity. These mechanisms include cell leakage, minimum inhibitory concentration, minimum killing concentration, and antibiofilm activity. The minimum inhibitory concentration (MIC) was determined with the agar diffusion method, the minimum biofilm forming concentration (MBC) was determined with the staining method, and cell leakage was determined with UV-VIS spectrophotometry at wavelengths of 260 nm (DNA) and 280 nm (protein). Additionally, the formation of anti-biofilms was determined with microtiter plates and gentian violet staining. In the moderate category, the MIC value tests showed a concentration of 0.3125% with an inhibition zone value of 6.80 0.05 mm. These results were obtained from the MIC value. Because there was no detectable bacterial growth on the streaked medium, KBM was obtained at a concentration of 10%. At wavelengths of 260 and 280 nanometers, leakage cells show absorbance absorption. The percentage of biofilm activity that is measured when the concentration is increased to 25% is 44.82%. In conclusion, the ethanol extract of basil leaves possesses a possible antibacterial mechanism against Salmonella typhi. This was discovered through testing.
Downloads
References
Amalia, A., Sari, I., & Nursanty, R. (2018). Aktivitas antibakteri ekstrak etil asetat daun sembung (Blumea balsamifera (L.) DC.) terhadap pertumbuhan bakteri Methicillin Resistant Staphylococcus aureus (MRSA). Prosiding Seminar Nasional Biotik, 5(1).
Berlian, Z., Aini, F., & Lestari, W. (2016). Aktivitas antifungi ekstrak daun kemangi (Ocimum americanum L.) terhadap fungi Fusarium oxysporum Schlecht. Jurnal Biota, 2(1), 99–105.
Christoper, W., Natalia, D., & Rahmayanti, S. (2018). Uji aktivitas antijamur ekstrak etanol umbi bawang dayak (eleutherine americana (aubl.) merr. Ex k. Heyne.) terhadap trichophyton mentagrophytes secara in vitro. Jurnal Kesehatan Andalas, 6(3), 685–689.
Dalimunthe, A., Pertiwi, D., Muhmmad, M., Kaban, V. E., Nasri, N., & Satria, D. (2022). The effect of extraction methods towards antioxidant activity of ethanol extract of Picria fel-terrae Lour. Herbs. IOP Conference Series: Earth and Environmental Science, 1115(1), 012040.
Davis, W. W., & Stout, T. R. (1971). Disc plate method of microbiological antibiotic assay: II. Novel procedure offering improved accuracy. Applied Microbiology, 22(4), 666–670.
Ditjen, P. O. M. (1979). Farmakope Indonesia Edisi III. DEPKES RI. Jakarta.
Haqi, H. D. (2018). AKTIVITAS ANTIBAKTERI EKSTRAK METANOL SERBUK BIJI KLUWIH (Artocarpus communis JR & G) TERHADAP PERTUMBUHAN Methicillint Resistant Staphylococcus aureus (MRSA) [PhD Thesis]. Universitas Muhammadiyah Semarang.
Imara, F. (2020). Salmonella typhi bakteri penyebab demam tifoid. Prosiding Seminar Nasional Biologi, 6(1), 1–5.
Kuspradini, H., Putri, A. S., Egra, S., & YANTI, Y. (2019). In vitro antibacterial activity of essential oils from twelve aromatic plants from East Kalimantan, Indonesia. Biodiversitas Journal of Biological Diversity, 20(7).
Larasati, D. A., & Apriliana, E. (2016). Efek Potensial Daun Kemangi (Ocimum basilicum L.) sebagai Pemanfaatan Hand Sanitizer. Jurnal Majority, 5(5), Article 5.
Lay, W. B. (1994). Analisis mikroba di laboratorium (Jakarta: PT. Raja Grafindo Persada).
Lubis, M. F., Syahputra, H., Illian, D. N., & Kaban, V. E. (2022). Antioxidant activity and nephroprotective effect of Lansium parasiticum leaves in doxorubicin-induced rats.
Muhammad, M., Nasri, N., Kaban, V. E., Satria, D., & Cintya, H. (2022). Antibacterial Potential Ethanol Extract of Papaya Leaves (Carica papaya Linn.) Towards Salmonella typhi. BEST Journal (Biology Education, Sains and Technology), 5(2), 265–270.
Musdja, M. Y. (2012). Efek Imunomodulator, Aktivitas Antibakteri Bahan dan Campuran Bahan Menyirih serta Perbandingan Komposisi Minyak Atsiri Daun Sirih dengan Campuran Bahan Menyirih.
Nasri, N., Harahap, U., Silalahi, J., & Satria, D. (2021). Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria. Biodiversitas Journal of Biological Diversity, 22(8).
Nasri, N., Kaban, V. E., Gurning, K., Syahputra, H. D., & Satria, D. (2022). Aktivitas Antibakteri Ekstrak Etanol Daun Pepaya (Carica papaya Linn.) Terhadap Bakteri Pseudomonas aeruginosa. INSOLOGI: Jurnal Sains Dan Teknologi, 1(3), Article 3. https://doi.org/10.55123/insologi.v1i3.438
Nasri, N., Kaban, V. E., Syahputra, H. D., & Satria, D. (2022). Aktivitas Antibakteri Ekstrak Etanol Daun Alpukat (Persea americana Mill) Terhadap Escherichia coli, Salmonella typhi, dan Pseudomonas aeruginosa. Herbal Medicine Journal, 5(1), 13–19.
Negara, K. S. (2014). Analisis implementasi kebijakan penggunaan antibiotika rasional untuk mencegah resistensi antibiotika di RSUP Sanglah Denpasar: Studi kasus infeksi methicillin resistant Staphylococcus aureus. Jurnal Administrasi Rumah Sakit Indonesia, 1(1).
Press, U. G. M. (2022). Membangun Inovasi di Era Pandemi. UGM PRESS.
Purba, D., & Aritonang, E. (2022). Pemeriksaan Hemoglobin Pada Penderita Demam Tifoid Di RS Islam Malahayati Medan Tahun 2022. JURNAL TEKNOLOGI KESEHATAN DAN ILMU SOSIAL (TEKESNOS), 4(1), 187–191.
Purba, I. E., Wandra, T., Nugrahini, N., Nawawi, S., & Kandun, N. (2016). Program pengendalian demam tifoid di Indonesia: Tantangan dan peluang. Media Penelitian Dan Pengembangan Kesehatan, 26(2), 99–108.
Rani, Z., Nasution, H. M., Kaban, V. E., Nasri, N., & Karo, N. B. (2022). Antibacterial activity of freshwater lobster (Cherax quadricarinatus) shell chitosan gel preparation against Escherichia coli and Staphylococcus aureus. Journal of Applied Pharmaceutical Science, Notice: Undefined offset: 3 in/home/japsonli/public_html/abstract. php on line 193.
Regina, F. (2022). KUALITAS DNA MEMBRAN CANGKANG ℡UR MALEO (Macrocephalon maleo) BERDASAR VARIASI METODE ISOLASI DNA [PhD Thesis]. Universitas Atma Jaya Yogyakarta.
Sari, N. P., Rustandi, Y., & Warnaen, A. (2022). Inovasi materi penyuluhan pembuatan pupuk organik cair (POC) dengan meminimalisir bau amoniak [PhD Thesis]. Polbangtan Malang.
Satria, D., Sofyanti, E., Wulandari, P., Pakpahan, S. D., & Limbong, S. A. (2022). Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC® 25175TM and Staphylococcus aureus ATCC® 6538TM. Pharmacia, 69(1), 195–202.
Siregar, A. F., Sabdono, A., & Pringgenies, D. (2012). Potensi Antibakteri Ekstrak Rumput Laut Terhadap Bakteri Penyakit Kulit Pseudomonas aeruginosa, Staphylococcus epidermidis, dan Micrococcus luteus. Journal of Marine Research, 1(2), Article 2.
Sophian, A., & Yustina, Y. (2023). Analisis Nilai Kemurnian DNA Menggunakan Nano Fotometer pada Rasio 260/230 yang Diisolasi dari Produk Nugget. Muhammadiyah Journal of Nutrition and Food Science (MJNF), 3(2), 82–86.
Syahputra, H. D., Nasri, N., & Kaban, V. E. (2022). Pengujian Potensi Aktivitas Antibakteri dari Daun Cep-cepan (Saurauia cauliflora DC.) dalam Formulasi Sediaan Gel Terhadap Propionibacterium acnes. Herbal Medicine Journal, 5(1), 28–32.
Syam, S. (2022). Etnobotani Tumbuhan Hutan sebagai Obat Tradisional oleh Masyarakat Desa Ratte Kabupaten Polewali Mandar. Pangale: Journal of Forestry and Environment, 2(2), 21–32.
Vijayakone, B. (2012). MANFAAT PENATALAKSANAAN DIABETES MELLITUS DENGAN PENGGUNAAN DAUN KEMANGI (Ocimum sanctum Linn.) DAN DAUN LIDAH BUAYA (Aloe barbadensis Mill.). Students e-Journal, 1(1), Article 1.
Widiarthi, N. P. F. O., Ratnayani, K., & Wirajana, I. N. (2014). PENGARUH PENAMBAHAN SUSU SKIM TERHADAP HASIL DNA METAGENOMIK DIISOLASI DARI TANAH HUTAN MANGROVE.
Bila bermanfaat silahkan share artikel ini
Berikan Komentar Anda terhadap artikel Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi
Pages: 79-84
Copyright (c) 2023 Nasri Nasri, Vera Estefania Kaban, Denny Satria, Hariyadi Dharmawan Syahputra, Zulmai Rani
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (Refer to The Effect of Open Access).
