Studi Kualitatif Aktivitas Amilolitik Bakteri Asam Laktat Yang Diisolasi Dari Pangan Tradisional Aceh Pliek U


  • Annisa Ammalia Kiti * Mail STIKES Assyifa Aceh, Indonesia
  • It Jamilah Universitas Sumatera Utara, Indonesia
  • Herla Rusmarilin Universitas Sumatera Utara, Indonesia
  • (*) Corresponding Author
Keywords: Enzyme; Amylolytic; Lactic Acid Bacteria; Pliek U

Abstract

Amylase is one of  the most essential enzymes that have potentially widely used in various industrial fields such as food, fermentation, and pharmacy. Amylase can be obtained from diversified organisms such as plants, animals and microbes. However, the main sources of these enzymes are bacteria and fungi that have dominated utilization in industrial sectors. This study was aimed to obtain the potential for amylolytic lactic acid bacteria (LAB) isolated from pliek u Acehnese traditional food. Amylolytic LABs were isolated with starch plate method and tested with Lugol's iodine reagent to measured the amylolytic index. The isolation results showed that 5 amylolytic potential LAB isolates were found with cells morphological characters  were bacilli, Gram positive and motile. Amylolytic index of five isolates were varied, the highest index was found in UKA5 isolate which was 11 mm while the lowest was found in UKA2 isolate which was 7 mm.

References

Agati, V., Guyot, J. P., Morlon‐Guyot, J., Talamond, P., & Hounhouigan, D. J. 1998. Isolation and characterization of new amylolytic strains of Lactobacillus fermentum from fermented maize doughs (mawe and ogi) from Benin. Journal of Applied Microbiology, 85 (3) : 512-520.

Aiyer, P. V. 2005. Amylases and their applications. African journal of biotechnology, 4(13): 1525-1529.

Apun, K., Jong, B. C., & Salleh, M. A. 2000. Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste. The Journal of General and Applied Microbiology, 46 (5) : 263-267.

Ashwini, K., Gaurav, K., Karthik, L., & Bhaskara Rao, K. V. 2011. Optimization, production and partial purification of extracellular α-amylase from Bacillus sp. marini. Arch Appl Sci Res, 3(1), 33-42.

Cappucino, J.G dan N. Sherman. 1999. Microbiology a laboratory manual. Fifth edition. California, The Benjamin/Cummings Science Publishing.

Díaz-Ruiz, G., Guyot, J., Ruiz-Teran, F., Morlon-Guyot, J., & Wacher, C. 2003. Microbial and physiological characterization of weakly amylolytic but fast-growing lactic acid bacteria: a functional role in supporting microbial diversity in pozol, a Mexican fermented maize beverage. Appl. Environ. Microbiol., 69(8) : 4367-4374.

Dutta, P., Akash, D., dan Sukanta, M. 2016. Optimization of the Medium for the Production of Extracellular Amylase by the Pseudomonas stutzeri ISL B5 Isolated from Municipal Solid Waste. Int. J.l of Microbiol, :1-7.

Fachrial, E., dan Harmileni, H. 2018. Aktivitas Antimikroba Dan Identifikasi Molekuler Bakteri Asam Laktat Yang Diisolasi Dari “Pliek U”, Makanan Fermentasi Tradisional Asal Aceh, Indonesia. In Seminar Nasional Sains dan Teknologi Informasi (SENSASI,) 1 (1) : 82-87.

Giraud, E., Brauman, A., Keleke, S., Lelong, B., & Raimbault, M. 1991. Isolation and physiological study of an amylolytic strain of Lactobacillus plantarum. Applied microbiology and biotechnology, 36(3), 379-383.

Jalma, M. D. dan Zachreini, I. 2016. Efektivitas Hambatan Senyawa Ekstrak Kasar Pliek U (Patarana) terhadap Pertumbuhan Salmonella typhi in vitro. Cermin Dunia Kedokteran, 43(6) : 407-410.

Kusumaningrum, K., Yusmarini, Y., & Ali, A. 2015. Isolasi dan Identifikasi Bakteri Asam Laktat Amilolitik dari Industri Pengolahan Pati Sagu. Jurnal Online Mahasiswa Fakultas Pertanian Universitas Riau, 2(1), 1-11.

Mishra, S., & Behera, N. 2008. Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes. African Journal of Biotechnology, 7(18).

Novita, R., Munira, M., & Hayati, R. 2017. Formulasi Sediaan Salep Ekstrak Etanol Pliek U Sebagai Antibakteri. AcTion: Aceh Nutrition Journal, 2(2) : 103-108.

Nurliana. 2009. Prospek makanan tradisional aceh sebagai makanan kesehatan: eksplorasi senyawa antimikrob dari minyak pliek u dan pliek u. Disertasi. Prodi Sains Veteriner, Sekolah Pascasarjana, Bogor, IPB.

Oluwadamilare, L.A., Odutayo, E. O., Ametefe, George, D., dan Farinu, A.O. 2019. A review of literature on isolation of bacteria α-amylase. International Journal of Engineering and Technology, 6 (1) : 1333-1341.

Olympia, M., Fukuda, H., Ono, H., Kaneko, Y., & Takano, M. 1995. Characterization of starch-hydrolyzing lactic acid bacteria isolated from a fermented fish and rice food,“Burong Isda”, and its amylolytic enzyme. Journal of Fermentation and Bioengineering, 80(2) : 124-130.

Petrova, P., Petrov, K., & Stoyancheva, G. 2013. Starch‐modifying enzymes of lactic acid bacteria–structures, properties, and applications. Starch‐Stärke, 65(1‐2) : 34-47.

Putri, W. D. 2011. Karakteristik Bakteri Asam Laktat Amilolitik Lokal dan Peranannya Dalam Baking Behaviour Pati Kasava Asam (Doctoral dissertation, Universitas Gadjah Mada).

Putri, W. D. R., Haryadi, D. W. M., & Cahyanto, M. N. 2012. Isolasi dan karakterisasi bakteri asam laktat amilolitik selama fermentasi growol, makanan tradisional Indonesia. Jurnal Teknologi Pertanian, 13(1), 52-60.

Pandey, A., Nigam, P., Soccol, C. R., Soccol, V. T., Singh, D., & Mohan, R. 2000. Advances in Microbial Amylases. Biotechnology and Applied Biochemistry, 31 (2) :135-152.

Mehta, D. and Tulasi, S. 2016. Bacterial and Archaeal -Amylases: Diversity and Amelioration of the Desirable Characteristics for Industrial Applications. Front. Microbiol, 7 (1129) : 1-21.

Rabfiani, S. dan Hanafiah, M. 2002. Karakterisasi Awal Bakteriosin Produksi Bakteri Asam Laktat Yang Diisolasi Dari Pliek U. Jurnal Sain Veteriner, 20(1) : 30-34.

Ravindran, R. and Jaiswal A.K. 2016. Microbial Enzyme Production Using Lignocellulosic Food Industry Wastes as Feedstock: A Review. J. Bioengineering (Basel), 3 (4) : 1-22.

Raveendran, S., Binod P., Sabeela B.U., Amith A., Anil K.M., Aravind M., Sharrel R., and Ashok P. 2018. Applications of Microbial Enzymes in Food Industry. Food Technol. Biotechnol. 56 (1) : 16–30.

Reddy, N. S., Nimmagadda, A., and Rao, K. S. 2003. An Overview of the Microbial α-Amylase Family. African Journal of Biotechnology, 2 (12) : 645-648.

Rinaldi, R., Samingan, S., dan Iswadi, I. 2018. Isolasi dan Identifikasi Jamur pada Proses Pembuatan Pliek U. Prosiding Biotik, 3 (1) : 273-280.

Robinson, P.K. 2015. Enzymes: Principles and Biotechnoligical Applications. Essays Biochem., 59: 1-41.

Singh, R., Manoj, K., Anshumali, M., and Praveen, K.M. 2016. Microbial Enzyme: Industrial Progress in 21st Century. 3 Biotech, 6 (2) : 174.

Sudirman, L. I. 2009. Characterization Antimicrobes of Pliek U, A Traditional Spice of Aceh. HAYATI Journal of Biosciences, 16(1), 30-34.

Souza, P. M. D. 2010. Application of microbial α-amylase in industry-A review. Brazilian Journal of Microbiology, 41 (4) : 850-861.

Suryani, S., Nofiandi, D., Mukhtar, H., Siska, M., Dharma, A., & Nasir, N. 2017. Identifikasi Molekular Bakteri Asam Laktat Lactobacillus paracasei yang Ada Pada Lapisan Minyak VCO. Jurnal Katalisator, 2(2), 79-87.

Susilawati, I. O., Batubara, U. M., dan Riany, H. 2015. Analisis Aktivitas Enzim Amilase yang Berasal dari Bakteri Tanah di Kawasan Universitas Jambi. SEMIRATA, 4(1) : 359-367.


Bila bermanfaat silahkan share artikel ini

Berikan Komentar Anda terhadap artikel Studi Kualitatif Aktivitas Amilolitik Bakteri Asam Laktat Yang Diisolasi Dari Pangan Tradisional Aceh Pliek U

Article History
Submitted: 2020-01-29 Published: 2020-03-11
Section
Articles